LOCUS       CHPF-pGEn2              7530 bp    DNA     circular     20-SEP-2016
FEATURES             Location/Qualifiers
     CDS             129..1108
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_recomb     complement(3839..3867)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    6158..6699
                     /note="WPRE"
     misc_recomb     6120..6142
                     /label=attB2
                     /note="attB2 site"
                     /note="recognized as attB2"
     misc_feature    complement(6706..6920)
                     /note="BGH pA"
     misc_feature    complement(7008..7137)
                     /note="3'-ITR"
     misc_feature    2982..3056
                     /note="TCM signal sequence"
     misc_feature    3060..3083
                     /note="His tag"
     misc_feature    3084..3134
                     /note="AviTag"
     misc_feature    3135..3830
                     /note="Superfolder GFP"
     misc_feature    2976..2981
                     /note="Kozak seq"
     misc_feature    3868..3890
                     /note="TEV site"
     misc_feature    3891..6116
                     /note="OPTIMIZED CHPF coding region"
     vector          join(6128..7530,1..3857)
                     /source="pGEn2-DEST"
                     /type="Destination Vector"
     clone           3858..6127
                     /source="CHPF PDONR221 GENE  "
                     /type="Entry Clone"
     source          1..7530
                     /dnas_title="CHPF-pGEn2"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAgcg gccgcGCCAC cATGAGACTG CTGACCGCCC
     3001 TGTTCGCCTA CTTCATCGTG GCCCTGATCC TGGCCTTCAG CGTGTCCGCC AAGAGCATGC
     3061 ACCACCACCA TCACCATCAT CACATGAGCG GCCTGAACGA CATCTTCGAG GCCCAGAAAA
     3121 TCGAGTGGCA CGAGATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG
     3181 TGGAACTGGA CGGCGACGTG AACGGCCACA AGTTCTCTGT GCGGGGCGAG GGCGAGGGGG
     3241 ACGCCACAAA TGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGAAAG CTGCCCGTGC
     3301 CCTGGCCTAC CCTGGTCACA ACCCTGACCT ACGGCGTGCA GTGCTTCAGC AGATACCCCG
     3361 ACCACATGAA GCGGCACGAT TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAAGAAC
     3421 GGACCATCAG CTTCAAGGAC GACGGCACCT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG
     3481 GCGACACCCT GGTCAACCGG ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAACA
     3541 TCCTGGGCCA CAAGCTCGAG TACAACTTCA ACAGCCACAA CGTGTACATC ACCGCCGACA
     3601 AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGGCA CAACGTGGAA GATGGCAGCG
     3661 TGCAGCTGGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC
     3721 CCGACAATCA CTACCTGAGC ACCCAGAGCG TGCTGAGCAA GGACCCCAAC GAGAAGCGGG
     3781 ACCACATGGT GCTGCTGGAA TTTGTGACCG CCGCTGGCAT CACCCACGGC GAATTCgcAT
     3841 CAACAAGTTT GTACAAAAAA GCAGGCTctg aaaacttgta ctttcaaggc GAGGAACCTT
     3901 GTGGCCCTGG ACCTCCTCAA CCTGGCGATT CTGAACTGCC TCCTAGAGGC AACACAAACG
     3961 CCGCCAGACG GCCTAATTCT GTGCAGCCTG GCGCCGAGAG AGAAAAACCT GGCGCTGGCG
     4021 AAGGTGCCGG CGAGAATTGG GAACCTAGAG TGCTGCCTTA TCACCCCGCT CAGCCTGGAC
     4081 AGGCCGCCAA GAAAGCCGTG CGGACCAGAT ATATCAGCAC CGAGCTGGGC ATCAGACAGA
     4141 GACTGCTGGT GGCTGTGCTG ACCAGCCAGA CAACACTGCC TACACTGGGC GTCGCCGTGA
     4201 ATAGAACACT GGGCCACAGA CTGGAAAGAG TGGTGTTTCT GACAGGCGCC AGAGGTAGAA
     4261 GGGCTCCTCC TGGAATGGCC GTGGTCACCC TTGGAGAGGA AAGACCCATC GGACATCTGC
     4321 ATCTGGCCCT GAGACATCTG CTGGAACAGC ATGGCGACGA CTTCGACTGG TTCTTCCTGG
     4381 TGCCTGACAC CACCTATACA GAAGCCCACG GACTGGCCAG ACTGACAGGA CATCTGTCTC
     4441 TTGCCAGCGC CGCTCACCTG TATCTGGGCA GACCTCAGGA TTTCATCGGC GGCGAACCTA
     4501 CACCTGGCAG ATACTGTCAT GGCGGCTTTG GCGTGCTGCT GAGCAGAATG CTGCTGCAAC
     4561 AGCTGAGGCC ACACCTGGAA GGCTGCAGAA ATGACATCGT GTCCGCCAGA CCTGACGAGT
     4621 GGCTGGGCAG ATGTATTCTG GATGCCACAG GCGTGGGCTG TACAGGCGAT CATGAAGGCG
     4681 TGCACTACAG CCACCTGGAA CTGTCTCCAG GCGAACCTGT GCAAGAGGGC GATCCTCACT
     4741 TTAGAAGCGC CCTGACAGCT CACCCCGTCA GAGATCCTGT GCACATGTAC CAGCTGCACA
     4801 AGGCCTTTGC CAGAGCCGAG CTGGAACGGA CCTACCAAGA GATCCAAGAG CTGCAGTGGG
     4861 AGATCCAGAA CACCTCTCAT CTGGCCGTGG ATGGCGATAG AGCTGCTGCT TGGCCTGTGG
     4921 GAATCCCTGC TCCTTCTAGA CCTGCCAGCA GATTCGAGGT GCTGAGATGG GACTACTTCA
     4981 CCGAGCAGCA TGCCTTCAGC TGTGCCGATG GCTCTCCTAG ATGTCCTCTG AGAGGCGCCG
     5041 ATAGAGCCGA TGTGGCTGAT GTGCTTGGCA CAGCCCTGGA AGAACTGAAC AGACGGTATC
     5101 ACCCTGCTCT GAGACTGCAG AAACAGCAGC TGGTCAACGG CTACAGAAGA TTCGACCCTG
     5161 CCAGAGGCAT GGAGTACACC CTGGATCTGC AGCTGGAAGC CCTTACACCT CAAGGCGGAA
     5221 GAAGGCCCCT GACCAGAAGA GTGCAACTGC TGCGGCCTCT GAGCAGAGTG GAAATCCTGC
     5281 CTGTGCCTTA CGTGACCGAG GCCTCTAGAC TTACAGTGCT GCTGCCACTG GCCGCTGCCG
     5341 AAAGAGATCT GGCTCCTGGC TTTCTGGAAG CCTTTGCTAC AGCTGCCCTG GAACCTGGGG
     5401 ATGCAGCTGC CGCTCTTACT CTGCTGCTGC TGTATGAACC TCGGCAGGCT CAGAGAGTGG
     5461 CTCACGCTGA CGTTTTCGCC CCTGTGAAAG CCCATGTGGC CGAACTGGAA AGGCGGTTTC
     5521 CTGGCGCTAG AGTGCCTTGG CTGTCTGTGC AAACAGCCGC TCCATCTCCT CTGAGGCTGA
     5581 TGGACCTGCT GTCCAAGAAG CACCCTCTGG ATACCCTGTT CCTGCTGGCC GGACCTGATA
     5641 CCGTGCTGAC CCCTGACTTC CTGAACAGAT GCAGGATGCA CGCCATCAGC GGCTGGCAGG
     5701 CCTTCTTTCC AATGCACTTT CAGGCCTTCC ATCCTGCCGT GGCTCCTCCA CAAGGACCTG
     5761 GACCACCTGA GCTGGGAAGA GATACCGGCA GATTTGACAG ACAGGCTGCC AGCGAGGCCT
     5821 GCTTCTACAA CTCTGATTAT GTGGCCGCCA GGGGCAGACT GGCTGCCGCT TCTGAACAAG
     5881 AAGAAGAACT GCTGGAAAGC CTGGACGTGT ACGAGCTGTT CCTCCACTTT AGCAGCCTGC
     5941 ACGTGCTGAG AGCTGTGGAA CCAGCTCTGC TGCAGAGATA CAGAGCCCAG ACATGTAGCG
     6001 CCAGACTGTC CGAGGATCTG TACCACAGAT GCCTGCAGAG CGTGCTGGAA GGACTGGGCT
     6061 CTAGAACACA GCTGGCTATG CTGCTGTTCG AGCAAGAGCA GGGCAACAGC ACATAGGACC
     6121 CAGCTTTCTT GTACAAAGTG GTTGATAGCT TGGATCCAAT CAACCTCTGG ATTACAAAAT
     6181 TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC
     6241 TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT
     6301 GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG
     6361 CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG
     6421 TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC
     6481 CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT
     6541 GTTGTCGGGG AAGCTGACGT CCTTTCCATG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT
     6601 GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG
     6661 CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGA GATCTGCCTC GACTGTGCCT
     6721 TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT
     6781 GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG TCTGAGTAGG
     6841 TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA TTGGGAAGAC
     6901 AATAGCAGGC ATGCTGGGGA CTCGAGTTAA GGGCGAATTG CCGATTAGGA TCTTCCTAGA
     6961 GCATGGCTAC GTAGATAAGT AGCATGGCGG GTTAATCATT AACTACAAGG AACCCCTAGT
     7021 GATGGAGTTG GCCACTCCCT CTCTGCGCGC TCGCTCGCTC ACTGAGGCCG GGCGACCAAA
     7081 GGTCGCCCGA CGCCCGGGCT TTGCCCGGGC GGCCTCAGTG AGCGAGCGAG CGCGCAGCCT
     7141 TAATTAAGAG CTCATGGCGC GCCTAGGCCT TGACGGCCTT CCGCCAATTC GCCCTATAGT
     7201 GAGTCGTATT ACGTCGCGCT CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC
     7261 TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG
     7321 CGAAGAGGCC CGCACCGAAA CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGG
     7381 AGCGCCCTGT AGCGGCCACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT
     7441 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT
     7501 TTTAACAAAA TATTAACGCT TACAATTTAG
//

